An Integrated Microfluidic System Enabling Real-Time Autonomous Field Surveillance for Vector-Borne Pathogens
Abstract not provided.
Publications
SMART PHONE-ENABLED DIAGNOSTIC PLATFORM FOR DETECTION OF PATHOGEN NUCLEIC ACIDS
Abstract not provided.
Real-time autonomous surveillance for vector-borne pathogens
Abstract not provided.
Smart Trap Real-time autonomous biosurveillance for mosquito-borne pathogens
Abstract not provided.
INVESTIGATION OF CELL TRAJECTORIES IN CHAOTIC FLOW FIELDS GENERATED BY A HELICAL STATIC MICROMIXER FOR RAPID CROSSLINKING KINETICS
Abstract not provided.
Real-time Autonomous Biosurveillance for Vector-borne Viral Pathogens (SMART Traps)
Real-Time Autonomous Biosurveillance For Vectorborne Pathogens (Smart Traps)
Abstract not provided.
Real-time Autonomous Biosurveillance for Vector-borne Viral Pathogens (SMART Traps)
Investigation of cell trajectories in chaotic flow fields generated by a helical static micromixer for rapid crosslinking kinetics
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Transcription factor (TF) binding pattern in chromatin provide precise and comprehensive information about the cell state. However, current analysis methods such as chromatin immunoprecipitation (ChIP) does not readily facilitate in-vivo characterization required to study short lived chromatin complexes. Here we develop a microfluidic stop flow system which is capable of mixing bacterial cells with formaldehyde on a sub-second mixing residence time scale enabling us to probe the kinetics of TF bound chromatin intermediates.
Real-Time Autonomous Biosurveillance for Vector-Borne Viral Pathogens
Real-time Autonomous Biosurveillance for Vector-borne Viral Pathogens
Automated Preparation of Nucleic Acids from Blood for Point-of-Care Applications
Abstract not provided.
The rotary zone thermal cycler: A low-power system enabling automated rapid PCR
PLoS ONE
Advances in molecular biology, microfluidics, and laboratory automation continue to expand the accessibility and applicability of these methods beyond the confines of conventional, centralized laboratory facilities and into point of use roles in clinical, military, forensic, and field-deployed applications. As a result, there is a growing need to adapt the unit operations of molecular biology (e.g., aliquoting, centrifuging, mixing, and thermal cycling) to compact, portable, low-power, and automation-ready formats. Here we present one such adaptation, the rotary zone thermal cycler (RZTC), a novel wheel-based device capable of cycling up to four different fixed-temperature blocks into contact with a stationary 4-microliter capillarybound sample to realize 1-3 second transitions with steady state heater power of less than 10 W. We demonstrate the utility of the RZTC for DNA amplification as part of a highly integrated rotary zone PCR (rzPCR) system that uses low-volume valves and syringe-based fluid handling to automate sample loading and unloading, thermal cycling, and between-run cleaning functionalities in a compact, modular form factor. In addition to characterizing the performance of the RZTC and the efficacy of different online cleaning protocols, we present preliminary results for rapid single-plex PCR, multiplex short tandem repeat (STR) amplification, and second strand cDNA synthesis.
Real-time Autonomous Biosurveillance for Vector-borne Viral Pathogens (SMART Traps)
A solvent replenishment solution for managing evaporation of biochemical reactions in air-matrix digital microfluidics devices
Lab on a Chip
Digital microfluidics (DMF) is a powerful technique for sample preparation and analysis for a broad range of biological and chemical applications. In many cases, it is desirable to carry out DMF on an open surface, such that the matrix surrounding the droplets is ambient air. However, the utility of the air-matrix DMF format has been severely limited by problems with droplet evaporation, especially when the droplet-based biochemical reactions require high temperatures for long periods of time. We present a simple solution for managing evaporation in air-matrix DMF: just-in-time replenishment of the reaction volume using droplets of solvent. We demonstrate that this solution enables DMF-mediated execution of several different biochemical reactions (RNA fragmentation, first-strand cDNA synthesis, and PCR) over a range of temperatures (4-95°C) and incubation times (up to 1 h or more) without use of oil, humidifying chambers, or off-chip heating modules. Reaction volumes and temperatures were maintained roughly constant over the course of each experiment, such that the reaction kinetics and products generated by the air-matrix DMF device were comparable to those of conventional benchscale reactions. This simple yet effective solution for evaporation management is an important advance in developing air-matrix DMF for a wide variety of new, high-impact applications, particularly in the biomedical sciences.
Advanced Diagnostic and Sample Preparation Platform for Early Threat Surveillance
Abstract not provided.
Reflected beam illumination microscopy using a microfluidics device (Progress report)
The document is a progress report on a first generation rapid testing microfluidic device with integrated sidewall mirrors being conducted by the Sandia National Laboratories team.
A World-to-Digital Microfluidic Interface for Whole Blood RNA Preparation
Abstract not provided.
A Microfluidic DNA Library Preparation Platform for Next-Generation Sequencing
PLoS ONE
Next-generation sequencing (NGS) is emerging as a powerful tool for elucidating genetic information for a wide range of applications. Unfortunately, the surging popularity of NGS has not yet been accompanied by an improvement in automated techniques for preparing formatted sequencing libraries. To address this challenge, we have developed a prototype microfluidic system for preparing sequencer-ready DNA libraries for analysis by Illumina sequencing. Our system combines droplet-based digital microfluidic (DMF) sample handling with peripheral modules to create a fully-integrated, sample-in library-out platform. In this report, we use our automated system to prepare NGS libraries from samples of human and bacterial genomic DNA. E. coli libraries prepared on-device from 5 ng of total DNA yielded excellent sequence coverage over the entire bacterial genome, with >99% alignment to the reference genome, even genome coverage, and good quality scores. Furthermore, we produced a de novo assembly on a previously unsequenced multi-drug resistant Klebsiella pneumoniae strain BAA-2146 (KpnNDM). The new method described here is fast, robust, scalable, and automated. Our device for library preparation will assist in the integration of NGS technology into a wide variety of laboratories, including small research laboratories and clinical laboratories. © 2013 Kim et al.
Automated DNA Extraction and Amplification for Portable Forensic Analysis
Abstract not provided.
A Brief Overview of Research Into Radiation Source Development and Radiation Sensors in the Microfabrication Program at Sandia National Laboratories
Abstract not provided.
Characterization of Pathogens in Clinical Specimens via Suppression of Host Background for Efficient Second Generation Sequencing Analyses
Abstract not provided.
Capillary PCR and Chip Electrophoresis for Rapid Detection of Carbapenamese genes in Klebsiella pneumoniae
Abstract not provided.
Copy of Automated Molecular Biology Platform Enabling Rapid & Efficient SGS Analysis of Pathogens in Clinical Samples
Abstract not provided.
Automated Molecular Biology Platform Enabling Rapid & Efficient SGS Analysis of Pathogens in Clinical Samples
Abstract not provided.
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