Publications Details

FIELD-DEPLOYABLE MICROFLUIDIC IMMUNOASSAY DEVICE FOR PROTEIN DETECTION

Choi, Gihoon; Mangadu, Betty; Light, Yooli K.; Meagher, Robert M.

We present a field-deployable microfluidic immunoassay device in response to the need for sensitive, quantitative, and high-throughput protein detection at point-of-need. The portable microfluidic system facilitates eight magnetic bead-based sandwich immunoassays from raw samples in 45 minutes. An innovative bead actuation strategy was incorporated into the system to automate multiple sample process steps with minimal user intervention. The device is capable of quantitative and sensitive protein analysis with a 10 pg/ml detection limit from interleukin 6-spiked human serum samples. We envision the reported device offering ultrasensitive point-of-care immunoassay tests for timely and accurate clinical diagnosis.