Publications

Dose-Response

Gott, Katherine M.; Potter, Charles A.; Doyle-Eisele, Melanie; Lin, Yong; Wilder, Julie W.; Scott, Bobby R.

US homeland security concerns regarding the potential misuse of some radiation sources used in radiobiological research, for example, cesium-137 (137Cs), have resulted in recommendations by the National Research Council to conduct studies into replacing these sources with suitable X-ray instruments. The objective of this research is to compare the effectiveness of an X-RAD 320 irradiator (PXINC 2010) with a 137Cs irradiator (Gammacell-1000 Unit) using an established bone marrow chimeric model. Using measured radiation doses for each instrument, we characterized the dose–response relationships for bone marrow and splenocyte ablation, using a cytotoxicity-hazard model. Our results show that the X-RAD 320 photon energy spectrum was suitable for ablating bone marrow at the 3 exposure levels used, similar to that of 137Cs photons. However, the 320-kV X-rays were not as effective as the much higher energy γ rays at depleting mouse splenocytes. Furthermore, the 3 X-ray levels used were less effective than the higher energy γ rays in allowing the successful engraftment of donor bone marrow, potentially as a result of the incomplete depletion of the spleen cells. More defined studies are warranted for determining whether bone marrow transplantation in mice can be successfully achieved using 320-kV X-rays. A higher X-ray dose then used is likely needed for transplantation success.

Wilder, Julie W.

Abstract not provided.

Wilder, Julie W.

Abstract not provided.

Wilder, Julie W.

Abstract not provided.

Wilder, Julie W.

Abstract not provided.

Wilder, Julie W.; Mancini, Giulio M.; Wakabi, Timothy W.; Boggs, Susan E.

A survey was designed to query former Biorisk management (BRM) trainees in the East Africa region about their practices post-training and their perceived future training needs. A subset of those surveyed had been trained as BRM trainers. The survey was conducted to obtain a baseline of BRM practices that can serve as a benchmark for performance monitoring, to identify priorities for future BRM training and to gauge local BRM trainers' abilities to deliver effective training. The survey revealed that less than 50% of the respondents could identify evidence of a BRM system in their institute. Coaching and mentoring by BRM experts was identified as being of highest benefit to enable success as BRM practitioners. Local trainers reached 1538 trainees in the previous year and reported that trainings positively correlated with desired BRM behavior. Acknowledgements The authors wish to sincerely thank all of the former biorisk management trainees in East Africa who agreed to participate in this survey. Their candid and honest input was extremely insightful. We also thank Lora Grainger (06826) and Ben Brodsky (Manager, 06824) for careful and critical review of the report. We are grateful for the financial support of the Defense Threat Reduction Agency, Cooperative Biological Engagement Program.

Wilder, Julie W.; Mancini, Giulio M.; Wakabi, Timothy W.; Boggs, Susan E.

A survey was designed to query former Biorisk management (BRM) trainees in the East Africa region about their practices post-training and their perceived future training needs. A subset of those surveyed had been trained as BRM trainers. The survey was conducted to obtain a baseline of BRM practices that can serve as a benchmark for performance monitoring, to identify priorities for future BRM training and to gauge local BRM trainers' abilities to deliver effective training. The survey revealed that less than 50% of the respondents could identify evidence of a BRM system in their institute. Coaching and mentoring by BRM experts was identified as being of highest benefit to enable success as BRM practitioners. Local trainers reached 1538 trainees in the previous year and reported that trainings positively correlated with desired BRM behavior. Acknowledgements The authors wish to sincerely thank all of the former biorisk management trainees in East Africa who agreed to participate in this survey. Their candid and honest input was extremely insightful. We also thank Lora Grainger (06826) and Ben Brodsky (Manager, 06824) for careful and critical review of the report. We are grateful for the financial support of the Defense Threat Reduction Agency, Cooperative Biological Engagement Program.