Total Internal Reflection Fluorescence (TIRF) Microscopy
Dual-color, high resolution, multi-wavelength excitation and emission-capable TIRF used to image interfacial cellular interactions at real-time speeds with single molecule sensitivity. Also capable of localization-based superresolution imaging (STORM, PALM, etc.) for attaining sub-diffraction limited images with resolution better than 50nm.
FTIR, Raman, fluorescence, infrared, neutron/X-ray scattering and reflectivity, fluorescence and scanning probe imaging systems.
Multivariate curve resolution, augmented classical least squares, partial least squares, and linear discriminant analysis.
BSL-1 and BSL-2 laboratories with capabilities in flow cytometry, PCR, quantitative PCR, bioanalyzer, lyophilization, cell disruption (French press and sonifer), DNA and protein gel electrophoresis and imaging, spectroscopy (including Nanodrop and microplate reader), microscopy (stereoscopic, inverted light, and confocal fluorescence), and centrifugation (including ultracentrifugation).
Laboratory-scale photobioreactors including shaking incubators with photosynthetic light banks and gassing manifolds for air/CO2 delivery and chemostats with light banks for room temperature cultivation. Scale-up cultivation capabilities include two 175 gallon ponds in a greenhouse with central pivots for air/CO2 delivery and mixing and online monitoring of water temperature, air temperature, humidity in the greenhouse, culture pH, turbidity, and reflectivity.