skip to: onlinetools | mainnavigation | content | footer

Bioenergy & Defense

Bioenergy & Defense Technologies

Key Capabilities

Hyperspectral Imaging (HSI): 3D Confocal and 2D Line Scanning Fluorescence Microscopy

3D hyperspectral confocal fluorescence microscope operates with diffraction-limited spatial resolution (~250 nm in x and y dimensions and ~600 nm in z direction) collecting > 8300 full-emission spectra per second (512 wavelengths in the visible and near-infrared spectral regions, 490 nm to 800 nm) with the use of a Sandia-designed high-throughput imaging prism spectrometer. 2D hyperspectral imaging system with demonstrated capability of 0.8 μm spatial resolution that can monitor 560 spectral channels over the range from 480 to 900 nm.

Total Internal Reflection Fluorescence (TIRF) Microscopy

    Hyperspectral confocal fluorescence microscope

Dual-color, high resolution, multi-wavelength excitation and emission-capable TIRF used to image interfacial cellular interactions at real-time speeds with single molecule sensitivity. Also capable of localization-based superresolution imaging (STORM, PALM, etc.) for attaining sub-diffraction limited images with resolution better than 50nm.

Advanced Analytical Spectroscopy

FTIR, Raman, fluorescence, infrared, neutron/X-ray scattering and reflectivity, fluorescence and scanning probe imaging systems.

Quantitative Spectral Analysis Algorithms

Multivariate curve resolution, augmented classical least squares, partial least squares, and linear discriminant analysis.

Molecular Biology, Microbiology, Immunology, and Cell Culture Experimentation

BSL-1 and BSL-2 laboratories with capabilities in flow cytometry, PCR, quantitative PCR, bioanalyzer, lyophilization, cell disruption (French press and sonifer), DNA and protein gel electrophoresis and imaging, spectroscopy (including Nanodrop and microplate reader), microscopy (stereoscopic, inverted light, and confocal fluorescence), and centrifugation (including ultracentrifugation).

Algal Cultivation

Laboratory-scale photobioreactors including shaking incubators with photosynthetic light banks and gassing manifolds for air/CO2 delivery and chemostats with light banks for room temperature cultivation. Scale-up cultivation capabilities include two 175 gallon ponds in a greenhouse with central pivots for air/CO2 delivery and mixing and online monitoring of water temperature, air temperature, humidity in the greenhouse, culture pH, turbidity, and reflectivity.

    Algae pond in greenhouse


All images on this site are subject to copyright. Any use of these images requires written permission.

Bioenergy & Defense web site contact