Deputy Director of Microbial and Enzyme Discovery
- High-throughput screening of cellulolytic enzymes across a matrix of conditions (T, pH, ionic liquid) to identify enzymes for mixture optimization and to understand the protein features that impart ionic liquid tolerance
- Understanding the enzymes involved in enzymatic depolymerization and metabolism of lignin and combining these enzymes to generate defined lignin fragments
- Developing novel methods for recovery and recycle of enzymes
- Understanding the changes in enzyme kinetics resulting from the presence of an ionic liquid
- Engineering enzymes for improved IL tolerance
Kohler, A; Mills, M; Adams, P; Simmons, B; and K. Sale. 2017. Structure of aryl O-demethylase offers molecular insight into a catalytic tyrosine-dependent mechanism. Proceedings of the National Academy of Sciences.
Zeng, J; Mills, M; Simmons, B; Kent, M; and K Sale. 2017. “Understanding factors controlling depolymerization and polymerization in catalytic degradation of β-ether linked model lignin compounds by versatile peroxidase.” Green Chemistry. 19, 2145-2154. (doi: 10.1039/C6GC03379B)
Mills, MJL; Sale, KL; Simmons, BA; and PLA Popelier. 2017. “Rhorix: An interface between quantum chemical topology and the 3D graphics program blender.” Journal of Computational Chemistry. 5;38(29):2538-2552. (doi: 10.1002/jcc.25054)
McAndrew, R; Sathitsuksanoh, N; Mbughuni, M; Heins, R; Pererira, J; George, A; Sale, K; Fox, B; Simmons, B; and P Adams. 2016. “Structure and mechanism of NOV1, a resveratrol-cleaving dioxygenase.” Proceedings of the National Academy of Sciences. .
Liszka, M; Kang, A; Murthy Konda, N.S.C.N; Tran, K; Gladden, J; Singh, S; Keasling, J; Scown, C; Soon Lee, T; Simmons, B; and K Sale. 2016. “Switchable ionic liquids based on di-carboxylic acids for one-pot conversion of biomass to an advanced biofuel.”, Green Chemistry.18, 4012-4021. (doi:10.1039/C6GC00657D)
Pereira, J; Heins, R; Gall, D; McAndrew, R; Deng, K; Holland, K; Donohue, T; Noguera, D; Simmons, B; Sale, K; Ralph, J; and P Adams. 2016. “Structural and Biochemical Characterization of the Early and Late Enzymes in the Lignin β-Aryl Ether Cleavage Pathway from Sphingobium sp. SYK-6.”, The Journal of Biological Chemistry. (doi: )
Helmich, K; Pereria, J; Gall, D; Heins, R; McAndrew, R; Bingman, C; Deng, K; Holland, K; Noguera, D; Simmons, B; Sale, K; Ralph, J; Donohue, T; Adams, P; and G Philips. 2016. “Structural Basis of Stereospecificity in the Bacterial Enzymatic Cleavage of β-Aryl Ether Bonds in Lignin.”, The Journal of Biological Chemistry. (doi: )
Kent, M; Avina, I; Rader, N; Busse, M; George, A; Sathisuksanoh, N; Baidoo, E; Timlin, J; Giron, N; Celina, M; Martin, L; Polsky, R; Chavez, V; Huber, D; Keasling, J; Singh, S; Simmons, B; and K Sale. 2015. “Assay for lignin breakdown based on lignin films: insights into the Fenton reaction with insoluble lignin.” Green Chemistry. (doi: 10.1039/C5GC01083G)
Deng, K; Guenther, J; Gao, J; Bowen, B; Tran, Huu; Reyes-Ortiz, V; Cheng, X; Sathitsuksanoh, N; Heins, R; Takasuka, T; Bergeman, L; Geertz-Hansen, H; Deutsch, S; Loque, D; Sale, K; Simmons, B; Adams, P; Singh, A; Fox, B; and T Northen. “Development of a High Throughput Platform for Screening Glycoside Hydrolases Based on Oxime-NIMS.” Frontiers in Bioengineering and Biotechnology.
Gladden, J; Park, J; Bergmann, J; Reyes-Ortiz, V; D'haeseleer, P; Quirino, B; Sale, K; Simmons, B; and S Singer. 2014. “Discovery and characterization of ionic liquid-tolerant thermophilic cellulases from a switchgrass-adapted microbial community" Biotechnology for Biofuels. 7:15.
Chen, Z; Pereira, J; Liu, H; Tran, H; Hsu, N; Dibble, D; Singh, S; Adams, P; Sapra, R; Hadi, M; Simmons, B; and K Sale. 2013. “Improved Activity of a Thermophilic Cellulase, Cel5A, from Thermotoga maritima on Ionic Liquid Pretreated Switchgrass." PLoS One.
McAndrew, RP; Park, JI; Heins, RA; Reindl, W; Friedland, GD; D'haeseleer, P; Northen, T; Sale, K; Simmons, B; and PD Adams. 2013. “From Soil to Structure, a Novel Dimeric ?-Glucosidase Belonging to Glycoside Hydrolase Family 3 Isolated from Compost Using Metagenomic Analysis.” Journal of Biological Chemistry. 288(21):14985-92. (doi: 10.1074/jbc.M113.458356)
Chen, Z; Friedland, GD; Pereira, JH; Reveco, SA; Chan, R; Park, JI; Thelen, MP; Adams, PD; Arkin, AP; Keaslin, JD; Blanch, HW; Simmons, BA; Sale, KL; Chivian, D; and SR Chhabra. 2012. “Tracing determinants of dual substrate specificity in glycoside hydrolase family 5.” Journal of Biological Chemistry. 287(30):25335-43. (doi: 10.1074/jbc.M112.362640)
Park, JI; Steen, EJ; Burd, H; Evans, SS; Redding-Johnson, AM; Batth, T; Benke, PI; D'haeseleer, P; Sun, N; Sale, KL; Keaslin, JD; Lee, TS; Petzold, CJ; Mukhopadhyay, A; Singer, SW; Simmons, BA; and JM Gladden. 2012. “A Thermophilic Ionic Liquid-Tolerant Cellulase Cocktail for the Production of Cellulosic Biofuels.” PLoS ONE. 7(5):e37010. (doi: 10.1371/journal.pone.0037010)
Zhang, T; Datta, S; Eichler, J; Ivanova, N; Axen, S; Kerfeld, C; Chen, F; Kyrpides, N; Hugenholtz, P; Cheng, JF; Sale, K; Simmons, B; and E Rubin. 2012. “Identification of a haloalkaliphilic and thermostable cellulase with improved ionic liquid tolerance.” Green Chemistry. 13, 2083-2090. (doi: 10.1039/C1GC15193B)
Liu, H; Pereira, J; Adams, P; Sapra, R; Simmons, B; and K Sale. 2010. “Molecular simulations provide new insights into the role of the accessory immunoglobulin-like domain of Cel9A.” FEBS Letters.