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MicroChemLab™ biodetector

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Victoria VanderNoot, PhD.

Analytical Chemist

Senior Member of Technical Staff,
Biosystems Research Department

Biographical

I received both a B.Sc. in chemistry and a Ph.D. in analytical chemistry from Carleton University in Canada. I then moved to the University of Colorado Health Sciences Center, where I held a joint academic/industrial postdoctoral position that was sponsored by Monsanto Corporation. Afterwards, I took a two-year postdoctoral position at the University of Iowa working jointly between the Departments of Medicinal & Natural Product Chemistry and Chemical & Biochemical Engineering. I joined Sandia National Laboratories in 1998 and am currently a principal member of technical staff.

Current Research Interests

My research interests focus on biochemical and bioanalytical methods for environmental, biomedical, and biodefense applications with an emphasis on optical, microfluidic, and biosensor technologies. A key component of my work in recent years has focused on adapting microfluidic systems, including Sandia’s µChemLab™ platform, to fieldable devices that incorporate automated sample preparation to allow the devices to operate autonomously in remote locations.

I served as principal investigator (PI) in a cooperative research and development agreement with Tenix to develop the unattended water sensor (UWS), a fully automated, microfluidic system that monitors domestic water for protein toxins. We plan to extend the UWS’s capabilities to other water-relevant analytes, including microorganisms (e.g., E. coli, Cryptosporidia, and Giardia) and freshwater toxins, such as the microcystins. In addition, from August 2006 to July 2007, I received funding through the National Oceanic and Atmospheric Administration’s Cooperative Institute for Coastal and Estuarine Environmental Technology to develop electrophoretic methods for marine toxins.

I have been heavily involved with developing and testing technologies for aerosol- and aqueous-based monitoring for bacterial agents and toxins. This effort, which was funded by the U.S. Department of Defense (DoD), resulted in a fully automated platform for monitoring aerosol-borne microbial agents that participated in in situ testing of prototypes at DoD facilities.

Additional work has expanded on the theme of agent detection by developing methods for (1) quickly detecting spores by analyzing small acid-soluble proteins, (2) rapidly determining viability through the analysis of early germination de novo protein synthesis in a microfluidic approach, and (3) resolving intact spores and bacteria via electrophoretic methods.

I also served as co-PI on a project funded by the National Institutes of Health, or more specifically, the National Institute of Dental and Craniofacial Research. This project developed electrophoretic methods and the Integrated Microfluidic Platform for Oral Diagnostics (IMPOD), an integrated platform for determining disease biomarkers in saliva and other oral fluids.

Selected Publications and Presentations

• Fruetel, JA, JAA West, BJ Debusschere, K Hukari, TW Lane, HN Najm, J Ortega, RF Renzi, I Shokair, VA VanderNoot. (in press). Rapid identification of viruses using microfluidic protein profiling and Bayesian classification. Analytical Chemistry.
• VanderNoot, V, E Van Blarigan, D Curtis, P Lane, T Lane. (2008). HABLab: A microfabricated total analytical system (μtas) for detecting phytotoxins in water. 122nd AOAC Annual Meeting & Exposition. Dallas, TX, September 21–24.
• VanderNoot, V, B Haroldsen, W Einfeld, Y Fintschenko. (2008). Unattended water sensor for detection of toxins in water systems. Environmental & Water Resources Institute Currents 9:6–7.
• VanderNoot, V, Keynote Speaker. (2008). Micro-total analytical systems and other field-capable platforms: Challenges and considerations. 28th Annual Meeting AOAC International Pacific Northwest Section. Tacoma, WA, June 18–19.

• Pizarro, SA, P Lane, TW Lane, E Cruz, B Haroldsen, VA VanderNoot. (2007). Bacterial characterization using protein profiling in an integrated microchip separations platform. Electrophoresis 28:4697–4704.
• Stachowiak, JC, EE Shugard, BP Mosier, RF Renzi, PF Caton, SM Ferko, JL Van de Vreugde, DD Yee, BL Haroldsen, VA VanderNoot. (2007). Autonomous microfluidic sample preparation system for protein profile-based detection of aerosolized bacterial cells and spores. Analytical Chemistry 79:5763–5770.
• VanderNoot, V. (2007). Portable bioagent detection systems for national security applications. Department of Homeland Security, 2007 Homeland Security Conference. Monterey, CA, August 21–24.
• VanderNoot, V, D Curtis, P Lane, T Lane. (2007). Development of a portable and automated electrophoresis-based system for in-field toxin detection: HABLab. PacRim 2007, Pacific Rim Shellfish Conference. San Diego, CA, April 22–23.
• VanderNoot, V et al. (2006). Integrated and automated sample preparation systems for aerosol and water monitoring applications. 2nd National Conference on Environmental Sampling and Detection for Bio-Threat Agents. New York, NY, October 25–27.
• Fruetel, JA, RF Renzi, VA VanderNoot, J Stamps, BA Horn, JAA West, S Ferko, R Crocker, CG Bailey, D Arnold, B Wiedenman, W-Y Choi, D Yee, I Shokair, E Hasselbrink, P Paul, D Rakestraw, D Padgen. (2005). Microchip separations of protein biotoxins using an integrated handheld device. Electrophoresis 26:1144–1154.
• Renzi, RF, J Stamps, BA Horn, S Ferko, VA VanderNoot, JAA West, R Crocker, B Wiedenman, D Yee, and JA Fruetel. (2004). Handheld microanalytical instrument for chip-based electrophoretic separations of proteins. Analytical Chemistry 77:435–441.

• VanderNoot, VA, GS Chirica, J Cintron, JAA West J Fruetel, TW Lane, PF Caton, K Wally, BL Haroldsen. (2004). Detection of bacterial biowarfare agents with a proteomics-based analyzer. 2nd Joint Conference on Point Detection for Chemical and Biological Defense. Williamsburg, VA, March 1–5.
• VanderNoot, V, S Pizarro, J Fruetel, R Renzi, J Stamps. (2003). Phosphoprotein signatures for early pathogen exposure detection using µChemLab™. Mainstreaming Microfluidics. Boston, MA, May 15–16.
• VanRollins, M, VA VanderNoot. (2003). Simultaneous resolution of underivatized regioisomers and stereoisomers of arachidonate epoxides by capillary electrophoresis. Analytical Biochemistry 313:106–116.
• VanderNoot, V, B Wiedenman, Y Fintschenko, J Fruetel. (2003). Incorporation of sample preconcentration into the µChemLab™/CB platform for enhanced sensitivity. 16th International Symposium on Microscale Separations and Analysis. San Diego, CA, January 17–22.
• VanderNoot, VA, M VanRollins. (2002). Capillary electrophoresis of cytochrome P-450 epoxygenase metabolites of arachidonic acid. 1. Resolution of regioisomers. Analytical Chemistry 74:5859–5865.
• VanderNoot, VA, M VanRollins. (2002). Capillary electrophoresis of cytochrome P-450 epoxygenase metabolites of arachidonic acid. 2. Resolution of stereoisomers. Analytical Chemistry 74:5866–5870.
• Fruetel, JA, BA Horn, JAA West, JF Stamps, VA VanderNoot, MC Stoddard, RF Renzi, D Padgen. (2002). Results From µChemLab™/CB, a portable system for detecting chemical and biological warfare agents. µTAS 2002. Y Babu et al. (eds.) Kluwer Academic: Dordrecht. pp. 524–526.
• Renzi, RF, JF Stamp, RW Crocker, BJ Wiedenman, SM Ferko, BA Horn, D Yee, VA VanderNoot, JA West, JA Fruetel, Y Fintschenko, MC Stoddard. (2002). Engineered improvement of the generation-2 µChemLab biotoxin detector. µTAS 2002. Y Babu et al. (eds.) Kluwer Academic: Dordrecht. pp. 703–705.
• Pizarro, SA, JS Schoeniger, VA VanderNoot. (2002). Capillary-based separation microtechnologies for pattern profiling of phosphoproteins involved in T-cell signaling. Microtechnologies in Medicine and Biology 2002 (IEEE-EMBS) Proceedings. A Dittmar, D Beebe (eds.) IEEE, Inc.: Piscataway, NJ. pp. 433–436.
• Fruetel, J. VA VanderNoot, B Kirby, C Hasselbrink, T Shepodd. (2002). Polymeric coating protocol for µChemLab/CB. 15th International Symposium on Microscale Separations and Analysis. Stockholm, Sweden, April 13–18.
• VanderNoot, VA, E Keuhler, S Pizarro. (2002). Isoelectric focusing with laser-induced fluorescence detection of phosphoprotein signatures. 15th International Symposium on Microscale Separations and Analysis. Stockholm, Sweden, April 13–18.
• VanderNoot VA, G Hux, J Schoeniger, T Shepodd. (2001). Isoelectric focusing using electrokinetically generated pressure mobilization. µTAS 2001. A van den Berg et al. (eds.) Kluwer Academic: Amsterdam. pp. 127–128.
• Fruetel J, R Renzi, R Crocker, V VanderNoot, J Stamps, D Yee, D Rakestraw, P Paul, D Arnold, C Bailey. (2001). µChemLab/CB: Parallel channel analysis in an integrated microchip device. 14th International Symposium on Microscale Separations and Analysis. Boston, MA, January 13–18.
• Fruetel, J, R Crocker, V VanderNoot, S Ferko, J Stamps, I Shokair, D Yee, C Hasselbrink. (2000). Development of µChemlab/CB for detection of biotoxins. Proceedings of the First Joint Detection for Chemical and Biological Defense. pp. 131–139.
• VanderNoot, VA, J Volponi, S Hebbring, JS Schoeniger. (1999). Development and characterization of receptor-based stationary phases for capillary affinity electrochromatography. Federation of Analytical Chemistry and Spectroscopy Societies Conference. Vancouver, B.C., Canada, October 25–29.
• Caldwell EE, AM Andreasen, MA Blietz, JN Serrahn, V VanderNoot, Y Park, G Yu, RJ Linhardt, JM Weiler. (1999). Heparin binding and augmentation of C1 inhibitor activity. Archives of Biochemistry and Biophysics 361:215–222.
• Fath M, V VanderNoot, I Kilpelainen, T Kinnunen, H Rauvala, RJ Linhardt. (1999). Interaction of soluble and surface-bound heparin binding growth-associated molecule with heparin. FEBS Letters 454:105–108.
• Capila, I, VA VanderNoot, TR Mealy, BA Seaton, RJ Linhardt. (1999). Interaction of heparin with annexin V. FEBS Letters 446:327–330.
• Lai, EPC, A Farfara, VA VanderNoot, M Kono, B Polsky. (1998). Surface plasmon resonance sensors using molecularly imprinted polymers for sorbent assay of theophylline, caffeine and xanthine. Canadian Journal of Chemistry 76:265–273.
• Choe, JH, VA VanderNoot, RJ Linhardt, JS Dordick. (1998). Affinity-based reversed micellar extraction and separation (ARMES) to resolve structurally similar glycoproteins. AICHE Journal 44:2542–2548.
• VanderNoot VA, RE Hileman, JS Dordick, RJ Linhardt. (1998). Affinity capillary electrophoresis employing immobilized glycosaminoglycan to resolve heparin-binding peptides. Electrophoresis 19:437–441.
• VanderNoot VA, JS Dordick, M Van Rollins. (1997). Capillary electrophoresis for the enantiomeric and regioisomeric resolution of fatty acid epoxides in biological samples. Federation of Analytical Chemistry and Spectroscopy Societies Conference. Providence, RI, October 26–30.
• Choe, JH, VA VanderNoot, RJ Linhardt, JS Dordick. (1997). Parameters affecting the affinity-based reversed micellar extraction and separation of glycoproteins. Biotechnology Progress 13:440–445.
• Chakrabarti, CL, B Marchand, VA VanderNoot, J Walker, WH Schroeder. (1996). Development of a new method for direct determination of selenium associated with atmospheric particulate matter using chemical modifiers and graphite probe furnace atomic-absorption. Spectrochimica Acta Part B—Atomic Spectroscopy 51:155–163.
• VanderNoot VA, FA Fitzpatrick. (1995). Competitive binding assay of src homology domain 3 interactions between 5-lipoxygenase and growth factor receptor binding protein 2. Analytical Biochemistry 230:108–114.
• Berg DA, VA VanderNoot, RG Barradas, EPC Lai. (1994). In situ photoacoustic determination of thallium underpotential deposition on copper. Journal of Electroanalytical Chemistry 369:33–37.
• VanderNoot VA, EPC Lai. (1992). Determination of mercury (II) in dithizone-impregnated latex microparticles by photochromism-induced photoacoustic spectroscopy. Analytical Chemistry 64:3187–3190.
• VanderNoot VA, EPC Lai. (1991). Detecting surface plasmon resonance using photothermal deflection spectroscopy: development of an optical immunosensor. Spectroscopy 6:28–33.

For more information, contact: Victoria VanderNoot