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Research in Microfluidic Transport at Sandia National Laboratories

Effect of IR laser on cells

To rule out potential photo-induced cell damage during optical deflection, we investigated the response of mouse macrophage to brief exposures (< 4 ms) of focused 1064-nm laser light (9.6 W at the sample). We found no significant difference in viability, cell proliferation, activation state, and functionality between infrared-exposed and unexposed cells. Activation state was measured by the phosphorylation of ERK and nuclear translocation of NF-ΚΒ while functionality was assessed in a similar manner, but after a lipopolysaccharide challenge.

Effect of IR laser on cells
Characterization of ERK phosphorylation by flow cytometry and GFP-RelA nuclear translocation by fluorescence microscopy in IR-exposed cells. (A) Phosphospecific immunostaining followed by flow cytometry analysis shows that ERK remains unphosphorylated. (B) Fluorescence microscopy confirms that GFP-RelA is located in the cytosol. After an LPS challenge, ERK is phosphorylated (C) and nuclear translocation of GFP-RelA occurs (D).

Related Publication

Perroud, T. D.; Kaiser, J. N.; Sy, J. C.; Lane, T. W.; Branda, C. S.; Singh, A. K.; Patel, K. D. Microfluidic-Based Cell Sorting of Francisella tularensis Infected Macrophages using Optical Forces. Analytical Chemistry 80, 6365-6372 (2008).