Electrokinetic Trapping

Sandia National Laboratory, Livermore

We have developed a novel technique for concentrating proteins from dilute solutions using electrokinetic trapping, in which charged macromolecules are reversibly trapped in a microchannel packed with porous silica particles under an applied electric field.

This technique provides a simple, on-column method of preconcentration of proteins and other charged macromolecules that can be easily integrated with a miniaturized separation device and requires changes in neither solvent, buffer, nor flow direction. A model protein, ovalbumin, has been concentrated by over two orders of magnitude using this technique.

Upcoming Presentations

A.K. Singh, D.J. Throckmorton, B.J. Kirby "A Novel Miniaturized Protein Preconcentrator Based on Electric Field-Addressable Trapping," HPCE 2003, San Diego, CA.

References

A.K. Singh, D.J. Throckmorton, B.J. Kirby, A.P. Thompson "A Novel Miniaturized Protein Preconcentrator Based on Electric Field-Addressable Trapping and Release," in MicroTAS 2002, p. 347-349, 2002.