Biosystems Research and Development
Interrogate innate immune responses to both bacterial and viral pathogens on microfluidic lab-on-a-chip devices. Develop methods to make quantitative, as well as temporal measurements of host intracellular signaling events at the single cell level. Use available reagents and in-house engineered microfluidic devices as building blocks to design the next generation of detection/diagnostic assays.
Pharmacological Compound Screening
Search for pharmacological compounds capable of attenuating innate immune responses in anticipation of pathogen invasion by screening compound libraries targeted to key kinases involved in innate immunity.
Using automated fractionation methods to prepare biological samples for subsequent Biomarker discovery.
Nimisha Srivastava, J. S. Brennan, R. F. Renzi, M. Wu, S. S. Branda, Anup K. Singh and Amy E. Herr, "Microfluidically-unified cell challenge, preparation and flow cytometry allows automated phosphoprofiling of macrophage response to lipopolysaccharide", In Press, Analytical Chemistry
Thomas D. Perroud, Robert J. Meagher, Michael P. Kanouff, Ronald F. Renzi, Meiye Wu, Anup K. Singh and Kamlesh D. Patel, "Isotropically etched radial micropore for cell concentration, immobilization, and picodroplet generation", Lab on a Chip, 2009, vol. 9, pp. 507 - 515
Nimisha Srivastava, M. Wu, C. S. Branda, J. S. Brennan, A. E. Herr and A.K. Singh," Spatiotemporal Analysis of Innate Immunity Enabled by Intergrated Microfluidic Single-cell Preparation, Imaging & Flow Cytometry", Miniaturised Systems for Chemistry and Life Sciences, µTAS San Diego, (2008)
Thomas D. Perroud, Meiye Wu, Ronald F. Renzi, Anup K. Singh, and Kamlesh D. Patel," Micropore Formation Using Overlapped Etch Fronts in Integrated Devices for Cellular Analysis Miniaturised Systems for Chemistry and Life Sciences", uTAS 2008 Conference proceedings. (2008)
Anastasia Constantinescu, Meiye Wu, Orna Asher, and Ivan Diamond," cAMP-dependent protein kinase type I regulates ethanol-induced CRE-mediated gene expression via activation of CREB binding protein and inhibition of MAPK", J. Biol. Chem., Aug 2004; 10.1074
Andrew A. Bremer, Morris F. Tansky, Meiye Wu, Norman D. Boyd and Susan E. Leeman, "Direct evidence for the interaction of neurokinin A with the tachykinin NK1 receptor in tissue", European Journal of Pharmacology, Volume 423, Issues 2-3, 6 July 2001, Pages 143-147
Tong Yungsong, Fobian YM, Wu M, Boyd ND., "Conformational Probes for Elucidating the nature of Substance P binding to the NK-1 Receptor: Initial Efforts to Map the Phe7-Phe8 Region", Bioorg. Med. Chem. Lett., 1998, 8, 1679-1682.
2008- Present Ph.D.student in Biochemistry and Molecular Biology
University of California at Davis, Davis, CA
9/2001 M.A. in Molecular Pharmacology
5/1997 B.A. Double Major in Neuroscience and Biology