Anson V. Hatch, Ph.D.

My primary research focus is to realize comprehensive Lab-on-a-Chip approaches for rapid detection of biomolecules present in trace quantities within complex biofluids and matrixes such as blood, saliva or extracted cells. Our group is developing a number of on-chip capabilities including sample preparation, sample concentration, buffer exchange, mixing, chemical reactions, separation and quantitative fluorescence-based detection strategies. Key projects include the development of portable medical diagnostics for presymptomatic detection of disease, infection or intoxication. To realize self-contained deployable devices, systems engineering is emphasized. Additional projects are investigating microfluidic systems as research tools to understand infection and host-response and to facilitate rapid and low-cost biomarker discovery and verification. Specific technology advancements have included preconcentration, mixing and reactions at size-exclusion membranes integrated with gel electrophoretic separations on-chip for protein immunoassays or sizing by SDS PAGE. We have also developed microscale immobilized pH gradients for high resolution isoelectric focusing. My doctoral thesis work focused primarily on novel diffusion-based analysis of binding interactions enabled by low Reynolds number flow conditions within microchannels. Additional contributions were made in the area of on-chip valving and pumping with ferromagnetic fluids and anhydrous storage of proteins on-chip.

Projects

Portable microfluidic platform for rapid detection of biotoxins, Funded by National Institute of Allergies and Infectious Disease, National Institute of Health (Co-I)

Integrated Microfluidic System for Oral Diagnostics, Funded by National Institute of Dental and Craniofacial Research, National Institute of Health(Co-I)

Biomarker Discovery and Verification, Sandia Lab Directed R&D, (Co-PI)

Deployable Pathogen Diagnostic System, Sandia Lab Directed R&D, (PI)

Portable Diagnostics of Rickettsial Infection, Joint Sandia/UTMB Postdoctoral Training Program, (Sandia PI)

Peer Reviewed Publications

Sommer GJ, Singh AK and Hatch AV. "Enrichment and fractionation of proteins via microscale pore limit electrophoresis". Lab on a Chip, DOI: 10.1039/B901320B. Jun 2009.

Sommer GJ, Hatch AV. “Review: Isoelectric Focusing in Microfluidic Devices”. Electrophoresis, 30(5): 742-757. Mar 2009.

Meagher RJ, Hatch AV, Renzi RF, Singh AK. "An Integrated, Portable Platform for Sensitive and Rapid Detection of Biological Toxins". Lab on a Chip, 8(12): 2046-2053. Dec 2008.

Sommer GJ, Singh AK, Hatch AV, “On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients”. Analytical Chemistry, 80(9):3327-33. May 2008.

Herr AE, Hatch AV, Giannobile WV, Throckmorton DJ, Tran H, Brennan JS, Singh AK. “Integrated microfluidic platform for oral diagnostics”. Annals of the New York Academy of Sciences, 1098: 362-374 Mar 2007.

Herr AE, Hatch AV, Throckmorton DJ, Tran H, Brennan JS, Giannobile WV, Singh AK. “Microfluidic immunoassays as rapid saliva-based clinical diagnostics”. Proceedings of the National Academy of Sciences of the United States of America, 104 (13): 5268-5273 Mar 2007.

Hatch AV, Herr AE, Throckmorton DJ, Brennan JS, Singh AK. “Integrated preconcentration SDS-PAGE of proteins in microchips using photopatterned cross-linked polyacrylamide gels”. Analytical Chemistry, 78 (14): 4976-4984 Jul 2006.

Chang, H. N., Hatch, A. V., Hawkins, K. R. and Yager, P. “Lab-on-a-chip and fluorescence sensing on the microscale” , in Fluorescence Sensors and Biosensors, R. B. Thompson, ed., ISBN 0-8247-2737-1, CRC Press, Boca Raton, FL, c.400 pp (2005)

Garcia E, Kirkham JR, Hatch AV, Hawkins KR, Yager P. ”Controlled microfluidic reconstitution of functional protein from an anhydrous storage depot”. Lab on a Chip 4 (1): 78-82 2004.

Hatch AV, Garcia E, Yager P. “Diffusion Based Analysis of Molecular Interactions in Microfluidic Devices”. Proceedings of the IEEE, Special issue: Biomedical Applications for MEMS and Microfluidics, 92 (1): 126-139 Jan 2004.

Hatch AV, Kamholz AE, Holman G, Yager P, Bohringer KF. “A Ferrofluidic Magnetic Micropump”. Journal of Microelectromechanical Systems, 10 (2): 215-221 Jun 2001.

Hatch AV, Kamholz AE, Hawkins KR, Munson MS, Schilling EA, Weigl BH, Yager P. “Microfluidic Diffusion-Based Analysis: Immunoassay in a T-Sensor”. Nature Biotechnology, 19(5): 461-465 May 2001.

Hatch AV, Sano T, Misasi J, Smith CL. “Rolling circle amplification of DNA immobilized on solid surfaces and its application to multiplex mutation detection”. Genetic Analysis – Biomolecular Engineering, 15 (2): 35-40 Apr 1999.

Selected Presentations, Conference Proceedings

AV Hatch, RJ Meagher, YC Wang, GJ Sommer, AK Singh. “Rapid Portable Diagnostic Platform For Toxin/Pathogen Exposure Scenarios”, Chemical and Biological Defense Physical Science and Technology Conference, New Orleans, LA. Nov 2008

YC Wang, AK Singh and AV Hatch. “Ultra-Rapid Sample Preconcentration Under Slant Field Using High Aspect Ratio Nanoporous Membranes”, The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2008), San Diego, CA. (Oral) Oct 2008

GJ Sommer, AK Singh and AV Hatch. “Precise Microscale Polymer Gradients Applied to Isoelectric Focusing and Pore Limit Electrophoresis”, The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2008), San Diego, CA. Oct 2008

AV Hatch, RJ Meagher and AK Singh. “Portable Diagnostics of Health/Disease and Toxin Exposure”, 11th Annual Force Health Protection Conference, Albequerque NM, (Oral) Aug 2008

AV Hatch, RJ Meagher, DS Reichmuth, AE Herr, MB Sinclair, DM Haaland, AK Singh. “Hyperspectral & Spatial Multiplexing of Ultrasensitive Immunoassays for Detecting Toxin Exposure” The 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2007), Paris, France (Oral presented by Amy Herr) Oct 2007

Hatch AV, Singh AK. “Voltage-Gated Protein Transport Through Nanoporous Membranes”. The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2006). Tokyo, Japan. (Oral) Nov 2006

Herr AE, Hatch AV, Throckmorton DJ, Tran HM, Brennan JS, Giannobile WV, Singh AK. “Rapid On-Chip Immunoassays for Detection of Endogenous Disease Biomarkers in Saliva”. American Chemical Society National Meeting & Exposition, San Francisco, CA. Sept 2006

Hatch AV, Herr AE, Throckmorton DJ, Brennan JS, Giannobile WV, Singh AK. “On-Chip Preconcentration of Proteins for Picomolar Detection in Oral Fluids”. Micro Total Analysis Systems 2005. Boston, MA. Proceedings of µTAS 2005: 1042-1044, (Oral) Oct 2005

Hatch AV, Garcia E, Yager P. “Analysis of Molecular Binding to Human Serum Albumin Using a T-Sensor”. Micro Total Analysis Systems 2003. Squaw Valley, CA. Proceedings of µTAS 2003: 1215-1218. Oct 2003

Yager P, Hatch AV, Hawkins K, Chang H. “The Diffusion Immunoassay; A Rapid Assay in Microfluidic and Static Multi-well Formats”. SmallTalk 2002, San Diego, CA. Jul 2002

Yager P, Hatch AV, Cabrera C, Schilling E, Macounova K, Hawkins K, Kamholz A. “Development of Polymeric Laminate Microfluidic Devices for Microanalytical Systems”. CSC 2002, Vancouver BC. (Oral, Presented by Anson Hatch) Jun 2002

Hatch AV, Yager P. “Diffusion Immunoassay in Polyacrylamide Hydrogels”. Micro Total Analysis Systems 2001. Monterey, CA. Proceedings of the µTAS Symposium 2001: 571-572. Oct 2001

Weigl BH, Hatch AV, Kamholz A, Yager P,"Novel Immunoassay Formats for Integrated Microfluidic Circuits - Diffusion Immunoassays (DIA)", SPIE 2000, Proceedings of SPIE, 3912: 50-56. Jan 2000

Hatch AV, Weigl B, Zebert D, Yager P. “Immunoanalysis in a T-Sensor”. SPIE conference on microfluidics. Santa Clara, CA. Proceedings of SPIE - Microfluidic devices and Systems II, Vol 3877:169-172. (Oral), Sept 1999

Patents

“Use of liquid junction potentials for electrophoresis without applied voltage in a microfluidic channel”, US pat. #7,141,429

“Microscale diffusion immunoassay”, US pat. #6,541,213, April 2003

“Magnetically actuated fluid handling devices for microfluidic applications”, US pat. #6,415,821, Jul. 2002

“Magnetically actuated fluid handling devices for microfluidic applications”, US pat. #6,408,884, Jun. 2002

“Nucleic acid arrays and methods of synthesis”, US pat. #6,284,497, Sep. 2001

Contact Information

Biosystems Research Department
Sandia National Laboratories
MS 9291, PO Box 969
7011 East Avenue
Livermore, CA 94551-0969