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Ashtamurthy S Pawate, Ph.D.

 

Research Summary

I work on genomic annotation and metabolic pathway reconstruction of microorganisms for biofuels production. Our goal is to increase the efficiency of ethanol production by adding and/or deleting genes to alter metabolic pathways in ethanol-tolerant bacteria. My work involves the computational effort of reconstructing metabolic pathways and simulating metabolite fluxes to achieve maximum efficiency for ethanol production, and subsequent wet-lab experiments towards achieving that goal.

Appointments:

2008 – Present: Postdoctoral Fellow, Sandia National Laboratories, Livermore, CA

2006 – 2008: Postdoctoral Research Associate, University of Illinois at Urbana-Champaign

Publications:

Szundi, I., et al., Flash-photolysis of fully reduced and mixed-valence CO-bound Rhodobacter sphaeroides cytochrome c oxidase: heme spectral shifts. Biochemistry, 2007. 46(44): p. 12568-78.

Han, D., et al., Replacing Asn207 by aspartate at the neck of the D channel in the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides results in decoupling the proton pump. Biochemistry, 2006. 45(47): p. 14064-74.

Vakkasoglu, A.S., et al., Mutations which decouple the proton pump of the cytochrome c oxidase from Rhodobacter sphaeroides perturb the environment of glutamate 286. FEBS Lett, 2006. 580(19): p. 4613-7.

Branden, G., et al., Controlled uncoupling and recoupling of proton pumping in cytochrome c oxidase. Proc Natl Acad Sci U S A, 2006. 103(2): p. 317-22.

Siletsky, S.A., et al., Transmembrane charge separation during the ferryl-oxo -> oxidized transition in a nonpumping mutant of cytochrome c oxidase. J Biol Chem, 2004. 279(50): p. 52558-65.

Namslauer, A., et al., Redox-coupled proton translocation in biological systems: proton shuttling in cytochrome c oxidase. Proc Natl Acad Sci U S A, 2003. 100(26): p. 15543-7.

Pawate, A.S., et al., A mutation in subunit I of cytochrome oxidase from Rhodobacter sphaeroides results in an increase in steady-state activity but completely eliminates proton pumping. Biochemistry, 2002. 41(45): p. 13417-23.

 

Contact Information

Ashtamurthy S Pawate, Ph.D.
Biosystems Research Department
Sandia National Laboratories
MS 9291, P.O. Box 969
7011 East Avenue
Livermore, CA 94551-0969

apawat@sandia.gov
Phone: 925-294-6360
Fax: 925-294-3020